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A note about BMV image frame data

Some map challenge participants may be encountering difficulty processing the BMV individual frames data (not the original boxed out particle images). One has to work out the image processing scheme which is a part of the challenge. To help challengers understand the data, the BMV raw data providers have provided the following note:

New Publications

New open access articles about EMDataBank and EMDB access are now available online, in advance of publication in the upcoming January 2016 Nucleic Acids Research Database Issue.

Map Challenge FAQ

Below are compiled questions/answers regarding the map challenge that may be of interest to all of the participants.  We'll update this FAQ as needed.

Challenge Phase

1. Is the Cs listed in the target table (2.7) correct for the Apoferritin data?  No. There was an error in the script to generate the metadata requested for the challenge. The manufacturer specified Cs value for the Polara is 2.0 mm, but this has never been measured accurately for the instrument. In practice, this is an somewhat arbitrary fitting parameter that can be input during analysis of the raw data or refined during fitting. Followup question: What Cs do the defocus values provided in the particle stack refer to?  The provided defocus values assume Cs = 2.7. posted Nov 20, 2015, thanks to Niko Grigorieff for questions, and Chris Russo for answers

SDSC Gordon Info

Everyone who applied for SDSC Gordon compute time for the map challenge will receive an initial allocation of 22,000 service units per proposed target. Altogether 14 users plan to perform a total of 45 reconstructions. Some useful links:

Other things to be aware of:

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EMDataBank Validation Challenges are supported by NIH National Institute of General Medical Sciences

Please send your challenge questions, comments and feedback to challenges@emdatabank.org

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